Great Britq , in A New Intermediate of the Aldolase Reaction , the Pyruvaldehyde - Aldolase - Orthophosphate Complex 361
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چکیده
Fructose 1,6-bisphosphate aldolase from rabbit muscle forms by reaction with dihydroxytacetone phosphate a pyruvaldehyde-aldolase-orthophosphate complex that is in equilibrium with the eneamine intermediate. The new intermediate accumulates in two phases. The first one is practically complete in 40ms, and the second occurs with an apparent first-order rate constant of 4.6+0.5s-1. The new intermediate breaks down slowly with the release into the medium of pyruvaldehyde and Pi. The rate of the spontaneous release is higher at acidic than at neutral pH.
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تاریخ انتشار 2005